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KMID : 1123920050190041000
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Korean Journal of Oriental Physiology and Pathology
2005 Volume.19 No. 4 p.1000 ~ p.1010
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Comparison of Immunomodualtory Effects of Water-extracted Aconiti lateralis Preparata Radix, Zingiberis Rhizoma, Cinnamomi Cortex and Evodiae Fructus
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Son Gil-Hyun
Shin Sang-Woo
Kwon Young-Kyu
Kim Sang-Chan
Park Jong-Hyun
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Abstract
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This study was carried out to investigate the comparison of immunomodualtory effects of water-extracted Aconiti lateralis Preparata Radix(PR), Zingiberis Rhizoma(ZR), Cinnamomi Cortex(CC) and Evodiae Fructus(EF). The parameter examined to assess apparent immunomodulatory effect of the water-extracted PR, ZR, CC and EF included the regulation of Nitric oxide (NO). Also, ZR and EF represent the expression of Th1/Th2 type cytokine, the change of B cell phenotype. The water-extracted PR, ZR, CC and EF inhibited NO production and iNOS protein expression in LPS stimulated RAW 264.7 macrophage cells. In the Th1 and Th2 cytokine expression, the water-extracted ZR and EF induced IL-2, IFNr and IL-10 mRNA gene expression. Therefore, it seems that the water-extracted ZR and EF have a inducing effect of Th1 and Th2 type cytokines. In the Flow cytometry analysis, the water-extracted ZR and EF changed B cell phenotype (CD45R/B220), did NOT in PR and CC. The water-extracted PR, ZR, CC and EF have a reducing effect of immune suppression cause by Methotrexate (MTX), an agent of immune suppression. These results suggest that the immunomodulatory effects of the water-extracted ZR and EF may be, in part, associated with the inducing IL-2 and IFNr mRNA gene expression In and regulation of NO production in macrophage cells.
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KEYWORD
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Aconiti lateralis Preparata Radix, Zingiberis Rhizoma, Cinnamomi Cortex, Evodiae Fructus, Th1/Th2 cytokine, NO, Methotrexate
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